This project focuses on the Saccharomyces cerevisiae mismatch repair protein yMutLα. We use atomic force microscopy to image yMutLα under a variety of conditions (presence and absence of DNA and/or adenine nucleotide, varying salt concentrations) in order to understand the role yMutLα plays in mismatch repair.
Figure 1: 3-D Image of yMutLα in the absence of ATP and DNA. The inset shows a 2D 1x1 µm image. By varying the conditions, conformational changes in the protein are observed.
The Erie Lab
UNC Chapel Hill
Department of Chemistry
CB #3290
Kenan Labs B825
Chapel Hill, NC 27599
lab phone (919) 962-6371
